Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia
Por:
Brea, D, Agulla, J, Staes, A, Gevaert, K, Campos, F, Sobrino, T, Blanco, M, Davalos, A, Castillo, J and Ramos-Cabrer, P
Publicada:
8 jul 2015
Categoría:
Multidisciplinary
Resumen:
In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contralateral tissues (at least 3-fold, p < 0.05). Using COFRADIC, 5412 proteins were identified, with 72 showing a difference in expression. Apart from blood-related proteins (such as serum albumin), both techniques showed that the 70 kDa family of heat shock proteins were highly expressed in the peri-infarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealed that only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressed by the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c) is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarct tissue in the ischemic brain.
Filiaciones:
Brea, D:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Dept Neurosci, Cellular & Mol Neurobiol Res Grp, Badalona, Spain
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Dept Neurosci, Grp Recerca Neurociencies,IGTP, Badalona, Spain
Agulla, J:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
Univ Hosp Salamanca, Res Unit, Salamanca, Spain
Inst Hlth Sci Castilla & Leon, Salamanca, Spain
Staes, A:
Univ Ghent VIB, Dept Med Prot Res, B-9052 Ghent, Belgium
Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium
Gevaert, K:
Univ Ghent VIB, Dept Med Prot Res, B-9052 Ghent, Belgium
Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium
Campos, F:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
Sobrino, T:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
Blanco, M:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
:
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Dept Neurosci, Cellular & Mol Neurobiol Res Grp, Badalona, Spain
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Dept Neurosci, Grp Recerca Neurociencies,IGTP, Badalona, Spain
Castillo, J:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
Ramos-Cabrer, P:
Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Univ Clin Hosp, Clin Neurosci Res Lab,Neurol Dept,Neurovasc Area, Santiago De Compostela, Spain
Green Submitted, Green Published, gold
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