Lineage of origin in rhabdomyosarcoma informs pharmacological response


Por: Abraham, J, Nunez-Alvarez, Y, Hettmer, S, Carrio, E, Chen, HIH, Nishijo, K, Huang, ET, Prajapati, SI, Walker, RL, Davis, S, Rebeles, J, Wiebush, H, McCleish, AT, Hampton, ST, Bjornson, CRR, Brack, AS, Wagers, AJ, Rando, TA, Capecchi, MR, Marini, FC, Ehler, BR, Zarzabal, LA, Goros, MW, Michalek, JE, Meltzer, PS, Langenau, DM, LeGallo, RD, Mansoor, A, Chen, Y, Suelves, M, Rubin, BP and Keller, C

Publicada: 15 jul 2014
Resumen:
Lineage or cell of origin of cancers is often unknown and thus is not a consideration in therapeutic approaches. Alveolar rhabdomyosarcoma (aRMS) is an aggressive childhood cancer for which the cell of origin remains debated. We used conditional genetic mouse models of aRMS to activate the pathognomonic Pax3:Foxo1 fusion oncogene and inactivate p53 in several stages of prenatal and postnatal muscle development. We reveal that lineage of origin significantly influences tumor histomorphology and sensitivity to targeted therapeutics. Furthermore, we uncovered differential transcriptional regulation of the Pax3:Foxo1 locus by tumor lineage of origin, which led us to identify the histone deacetylase inhibitor entinostat as a pharmacological agent for the potential conversion of Pax3:Foxo1-positive aRMS to a state akin to fusion-negative RMS through direct transcriptional suppression of Pax3:Foxo1.

Filiaciones:
Abraham, J:
 Oregon Hlth & Sci Univ, Pape Family Pediat Res Inst, Pediat Canc Biol Program, Dept Pediat, Portland, OR 97239 USA

Nunez-Alvarez, Y:
 Germans I Trias Pujol Hlth Sci Res Inst IGTP, IMPPC, Badalona 08916, Spain

Hettmer, S:
 Harvard Univ, Howard Hughes Med Inst, Harvard Stem Cell Inst, Joslin Diabet Ctr,Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

 Childrens Hosp, Dana Farber Canc Inst, Dept Pediat Oncol, Div Pediat Hematol Oncol, Boston, MA 02115 USA

Carrio, E:
 Germans I Trias Pujol Hlth Sci Res Inst IGTP, IMPPC, Badalona 08916, Spain

Chen, HIH:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

Nishijo, K:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

Huang, ET:
 Oregon Hlth & Sci Univ, Pape Family Pediat Res Inst, Pediat Canc Biol Program, Dept Pediat, Portland, OR 97239 USA

Prajapati, SI:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

Walker, RL:
 Natl Canc Inst, Oncogen Sect, Pediat Oncol Branch, Adv Technol Ctr, Gaithersburg, MD 20877 USA

Davis, S:
 Natl Canc Inst, Oncogen Sect, Pediat Oncol Branch, Adv Technol Ctr, Gaithersburg, MD 20877 USA

Rebeles, J:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

Wiebush, H:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

McCleish, AT:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

Hampton, ST:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

Bjornson, CRR:
 Stanford Univ, Dept Neurol, Dept Neurol Sci, Glenn Labs Biol Aging, Palo Alto, CA 94304 USA

Brack, AS:
 Stanford Univ, Dept Neurol, Dept Neurol Sci, Glenn Labs Biol Aging, Palo Alto, CA 94304 USA

Wagers, AJ:
 Harvard Univ, Howard Hughes Med Inst, Harvard Stem Cell Inst, Joslin Diabet Ctr,Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

Capecchi, MR:
 Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA

Marini, FC:
 Wake Forest Univ, Bowman Gray Sch Med, Inst Regenerat Med, Winston Salem, NC 27157 USA

Ehler, BR:
 Univ Texas Hlth Sci Ctr San Antonio, Dept Epidemiol & Biostat, San Antonio, TX 78229 USA

Zarzabal, LA:
 Univ Texas Hlth Sci Ctr San Antonio, Dept Epidemiol & Biostat, San Antonio, TX 78229 USA

Goros, MW:
 Univ Texas Hlth Sci Ctr San Antonio, Dept Epidemiol & Biostat, San Antonio, TX 78229 USA

Michalek, JE:
 Univ Texas Hlth Sci Ctr San Antonio, Dept Epidemiol & Biostat, San Antonio, TX 78229 USA

Meltzer, PS:
 Natl Canc Inst, Oncogen Sect, Pediat Oncol Branch, Adv Technol Ctr, Gaithersburg, MD 20877 USA

Langenau, DM:
 Massachusetts Gen Hosp, Ctr Canc Res, Dept Pathol, Charlestown, MA 02129 USA

LeGallo, RD:
 Univ Virginia, Dept Pathol, Charlottesville, VA 22903 USA

Mansoor, A:
 Oregon Hlth & Sci Univ, Dept Pathol, Portland, OR 97239 USA

Chen, Y:
 Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA

 Univ Texas Hlth Sci Ctr San Antonio, Dept Epidemiol & Biostat, San Antonio, TX 78229 USA

:
 Germans I Trias Pujol Hlth Sci Res Inst IGTP, IMPPC, Badalona 08916, Spain

Rubin, BP:
 Cleveland Clin Fdn, Lerner Res Inst, Taussig Canc Ctr, Dept Anat Pathol,Dept Mol Genet, Cleveland, OH 44195 USA

Keller, C:
 Oregon Hlth & Sci Univ, Pape Family Pediat Res Inst, Pediat Canc Biol Program, Dept Pediat, Portland, OR 97239 USA
ISSN: 15495477





GENES & DEVELOPMENT
Editorial
Cold Spring Harbor Laboratory Press, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 28 Número: 14
Páginas: 1578-1591
WOS Id: 000339166100007
ID de PubMed: 25030697
imagen Green Published, gold

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