A Role for Oncostatin M in the Impairment of Glucose Homeostasis in Obesity
Por:
Piquer-Garcia, I, Campderros, L, Taxeras, SD, Gavalda-Navarro, A, Pardo, R, Vila, M, Pellitero, S, Martinez, E, Tarasco, J, Moreno, P, Villarroya, J, Cereijo, R, Gonzalez, L, Reyes, M, Rodriguez-Fernandez, S, Vives-Pi, M, Lerin, C, Elks, CM, Stephens, JM, Puig-Domingo, M, Villarroya, F, Villena, JA and Sanchez-Infantes, D
Publicada:
1 mar 2020
Resumen:
Context: Oncostatin M (OSM) plays a key role in inflammation, but its regulation and function during obesity is not fully understood.
Objective: The aim of this study was to evaluate the relationship of OSM with the inflammatory state that leads to impaired glucose homeostasis in obesity. We also assessed whether OSM immunoneutralization could revert metabolic disturbances caused by a high-fat diet (HFD) in mice.
Design: 28 patients with severe obesity were included and stratified into two groups: (1) glucose levels <100 mg/dL and (2) glucose levels >100 mg/dL. White adipose tissue was obtained to examine OSM gene expression. Human adipocytes were used to evaluate the effect of OSM in the inflammatory response, and HFD-fed C57BL/6J mice were injected with anti-OSM antibody to evaluate its effects.
Results: OSM expression was elevated in subcutaneous and visceral fat from patients with obesity and hyperglycemia, and correlated with Glut4 mRNA levels, serum insulin, homeostatic model assessment of insulin resistance, and inflammatory markers. OSM inhibited adipogenesis and induced inflammation in human adipocytes. Finally, OSM receptor knockout mice had increased Glut4 mRNA levels in adipose tissue, and OSM immunoneutralization resulted in a reduction of glucose levels and Ccl2 expression in adipose tissue from HFD-fed mice.
Conclusions: OSM contributes to the inflammatory state during obesity and may be involved in the development of insulin resistance.
Filiaciones:
Piquer-Garcia, I:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
Campderros, L:
Univ Barcelona, Dept Biochem & Mol Biomed, Barcelona, Spain
Univ Barcelona, Inst Biomed, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Obesidad & Nutr, Madrid, Spain
Taxeras, SD:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
Gavalda-Navarro, A:
Univ Barcelona, Dept Biochem & Mol Biomed, Barcelona, Spain
Univ Barcelona, Inst Biomed, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Obesidad & Nutr, Madrid, Spain
Pardo, R:
Univ Autonoma Barcelona, Vall dHebron Inst Recerca, Lab Metab & Obes, Barcelona, Spain
Vila, M:
Univ Autonoma Barcelona, Vall dHebron Inst Recerca, Lab Metab & Obes, Barcelona, Spain
:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Diabet & Enferme, Madrid, Spain
Martinez, E:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
Tarasco, J:
Germans Trias & Pujol Res Inst, Dept Surg, Barcelona, Spain
Moreno, P:
Germans Trias & Pujol Res Inst, Dept Surg, Barcelona, Spain
Villarroya, J:
Univ Barcelona, Dept Biochem & Mol Biomed, Barcelona, Spain
Univ Barcelona, Inst Biomed, Barcelona, Spain
Hosp Santa Creu & Sant Pau, Infect Dis Unit, Barcelona, Spain
Cereijo, R:
Univ Barcelona, Dept Biochem & Mol Biomed, Barcelona, Spain
Univ Barcelona, Inst Biomed, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Obesidad & Nutr, Madrid, Spain
:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
Reyes, M:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
:
Germans Trias & Pujol Res Inst, Immunol Sect, Barcelona, Spain
:
ISCIII, BiomedicalRes Ctr Red Fisiopatol Diabet & Enferme, Madrid, Spain
Germans Trias & Pujol Res Inst, Immunol Sect, Barcelona, Spain
Lerin, C:
St Joan Deu Hosp, Endocrinol, Barcelona, Spain
Elks, CM:
Pennington Biomed Res Ctr, Matrix Biol Lab, 6400 Perkins Rd, Baton Rouge, LA 70808 USA
Stephens, JM:
Pennington Biomed Res Ctr, Adipocyte Biol Lab, 6400 Perkins Rd, Baton Rouge, LA 70808 USA
:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Diabet & Enferme, Madrid, Spain
Villarroya, F:
Univ Barcelona, Dept Biochem & Mol Biomed, Barcelona, Spain
Univ Barcelona, Inst Biomed, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Obesidad & Nutr, Madrid, Spain
Villena, JA:
Univ Autonoma Barcelona, Vall dHebron Inst Recerca, Lab Metab & Obes, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Diabet & Enferme, Madrid, Spain
:
Germans Trias & Pujol Res Inst, Dept Endocrinol & Nutr, Barcelona, Spain
ISCIII, BiomedicalRes Ctr Red Fisiopatol Obesidad & Nutr, Madrid, Spain
Bronze, Green Published, Green Accepted
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