Laparoscopic sleeve gastrectomy induces molecular changes in peripheral white blood cells
Por:
Beisani, M, Pappa, S, Moreno, P, Martinez, E, Tarasco, J, Granada, ML, Puig, R, Cremades, M, Puig-Domingo, M, Jorda, M, Pellitero, S and Balibrea, JM
Publicada:
1 feb 2020
Ahead of Print:
19 mar 2019
Resumen:
Background & aims: Peripheral white blood cells (PWBC) may allow for the development of obesity biomarkers. We aimed to investigate the existence of gene expression and DNA methylation changes in PWBC after a very low calorie diet (VLCD) followed by a laparoscopic sleeve gastrectomy (LSG), and its correlation with surgical outcomes.
Methods: From July 2013 to June 2014, 35 consecutive bariatric patients and 33 healthy lean volunteers were recruited. Molecular data was obtained once on the control group and at 3 different times on the LSG group: 1) at baseline; 2) after 2 weeks of VLCD, right before LSG; and 3) 6 months after LSG. The expression of 12 genes in PWBC was analyzed by quantitative real-time polymerase chain reaction: ghrelin (GHRL), visfatin (NAMPT), insulin receptor substrate 1 (IRS1), fat mass and obesity-related gene (FTO), leptin (LEP), peroxisome proliferator-activated receptor gamma (PPARG), adiponectin (ADIPOQ), fatty acid synthase (FASN), melanocortin 4 receptor (MC4R), fas cell surface death receptor (FAS), tumor necrosis factor alpha (TNF) and chemokine (C-C motif) ligand 2 (CCL2). Moreover, DNA methylation of GHRL, NAMPT and FAS promoters was analyzed in PWBC by bisulfite pyrosequencing.
Results: Seven genes (GHRL, NAMPT, IRS1, FTO, FAS, TNF and CCL2) had detectable expression in PWBC. FTO expression at baseline was lower in patients than in controls (p = 0.042), equalizing after LSG. In patients, FAS expression decreased after VLCD (p = 0.01) and stayed low after LSG (p = 0.015). Also, CCL2 expression decreased 50% after LSG compared to pre-surgical levels (p = 0.016). All studied CpG sites in the GHRL gene promoter followed a consistent pattern of DNA methylationidemethylation. No direct correlation between these molecular changes and surgical outcomes was found at 1-year follow-up.
Conclusions: FTO expression increased and FAS and CCL2 expression decreased in PWBC after LSG. Molecular changes did not correlate with surgical outcomes. (C) 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
Filiaciones:
Beisani, M:
Hosp del Mar, Dept Surg, Passeig Maritim 25-29, Barcelona, Spain
CREBA, Lleida, Spain
Pappa, S:
Germans Trias & Pujol Res Inst PMPPC IGTP, Program Predict & Personalized Med Canc, Badalona, Spain
Moreno, P:
Germans Trias & Pujol Univ, Hosp & Res Inst, Dept Surg, Badalona, Spain
Martinez, E:
Germans Trias & Pujol Univ, Hosp & Res Inst, Univ Autonoma Barcelona, Endocrinol & Nutr Serv,Dept Med, Badalona, Spain
Tarasco, J:
Germans Trias & Pujol Univ, Hosp & Res Inst, Dept Surg, Badalona, Spain
:
Germans Trias & Pujol Univ, Hosp & Res Inst, Clin Biochem Serv, Badalona, Spain
:
Germans Trias & Pujol Univ, Hosp & Res Inst, Univ Autonoma Barcelona, Endocrinol & Nutr Serv,Dept Med, Badalona, Spain
Cremades, M:
Germans Trias & Pujol Univ, Hosp & Res Inst, Dept Surg, Badalona, Spain
:
Germans Trias & Pujol Univ, Hosp & Res Inst, Univ Autonoma Barcelona, Endocrinol & Nutr Serv,Dept Med, Badalona, Spain
Inst Salud Carlos III, CIBER Diabet & Enfermedades Metab Asociadas CIBER, Madrid, Spain
Inst Salud Carlos III, CIBER Enfermedades Raras CIBERER, Madrid, Spain
:
Germans Trias & Pujol Res Inst PMPPC IGTP, Program Predict & Personalized Med Canc, Badalona, Spain
:
Germans Trias & Pujol Univ, Hosp & Res Inst, Univ Autonoma Barcelona, Endocrinol & Nutr Serv,Dept Med, Badalona, Spain
Inst Salud Carlos III, CIBER Diabet & Enfermedades Metab Asociadas CIBER, Madrid, Spain
Balibrea, JM:
Univ Autonoma Barcelona, Vall dHebron Univ Hosp, EAC BS Ctr Excellence, Endocrine Metab & Bariatr Surg Unit, Barcelona, Spain
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