PrimeStore MTM and OMNIgene Sputum for the Preservation of Sputum for Xpert MTB/RIF Testing in Nigeria
Por:
Bimba, JS, Lawson, L, Kontogianni, K, Edwards, T, Ekpenyong, BE, Dodd, J, Adams, ER, Sloan, DJ, Creswell, J, Dominguez, J and Cuevas, LE
Publicada:
1 dic 2019
Ahead of Print:
4 dic 2019
Resumen:
Background: Xpert MTB/RIF (GX) for tuberculosis (TB) diagnosis is often located in reference laboratories, and sputum needs to be transported using a cold chain. Transport media to preserve sputum are available, but performance data under programmatic conditions are limited. Methods: Sputum samples were collected from patients with presumptive TB in Nigeria. One sputum was transported in a cold chain, tested immediately with GX and cultured. One sputum was swabbed and stored in PrimeStore-Molecular-Transport-Medium (Primestore), and the remainder was stored in OMNIGene-sputum (Omnigene), kept for seven days and tested with GX. Results: Of 248 patients, 63 were fresh-sputum culture-positive and 56 GX-positive (sensitivity 88.9%, 95% CI: 78.4-95.4%). Four of 185 culture-negative patients were GX-positive (specificity 97.8%, 94.6-99.4%). Omnigene GX and Primestore GX were positive in 56/62 (90.3%, 80.1-96.4%) and 49/62 (79.0%, 66.8-88.3%) culture-positive, respectively, and 1/185 (99.5%, 97.0-100.0%) and 3/185 (98.4%, 95.3-99.7%) were culture-negative patients. 14 Human Immunodeficiency Virus (HIV)-infected and 44 HIV-uninfected patients were culture-positive. Omnigene and Primestore detected 12/14 (85.7%, 57.2-98.2%) and 5/14 (35.7%, 12.8-64.9%) HIV-infected and 41/44 (93.2%, 81.3-98.6%) HIV-uninfected culture-positive patients. Interpretation: Omnigene stored and fresh sputum samples had similar GX results. The GX results of Primestore-stored samples were similar to those found in the fresh sputum of non-HIV infected patients, but GX-positivity was lower in HIV-infected patients. This was likely due to the lower amount of bacilli collected by the swab and transferred to PrimeStore.
Filiaciones:
Bimba, JS:
Bingham Univ, Zankli Res Ctr, Karu 961105, Nigeria
Bingham Univ, Dept Community Med, Karu 961105, Nigeria
Lawson, L:
Bingham Univ, Zankli Res Ctr, Karu 961105, Nigeria
Bingham Univ, Dept Community Med, Karu 961105, Nigeria
Zankli Med Ctr, Abuja 961105, Nigeria
Kontogianni, K:
Univ Liverpool Liverpool Sch Trop Med, Ctr Drugs & Diagnost, Liverpool L3 5QA, Merseyside, England
Edwards, T:
Univ Liverpool Liverpool Sch Trop Med, Ctr Drugs & Diagnost, Liverpool L3 5QA, Merseyside, England
Ekpenyong, BE:
Bingham Univ, Zankli Res Ctr, Karu 961105, Nigeria
Bingham Univ, Dept Community Med, Karu 961105, Nigeria
Dodd, J:
Univ Liverpool Liverpool Sch Trop Med, Dept Clin Sci, Liverpool L3 5QA, Merseyside, England
Adams, ER:
Univ Liverpool Liverpool Sch Trop Med, Ctr Drugs & Diagnost, Liverpool L3 5QA, Merseyside, England
Sloan, DJ:
Univ St Andrews, Sch Med, St Andrews KY16 9TF, Fife, Scotland
Creswell, J:
TB REACH, Stop TB Partnership, CH-1218 Geneva, Switzerland
:
Univ Autonoma Barcelona, CIBER Enfermedades Respiratorias CIBERES, Inst Invest Germans Trias & Pujol, Badalona 08916, Spain
Cuevas, LE:
Univ Liverpool Liverpool Sch Trop Med, Ctr Drugs & Diagnost, Liverpool L3 5QA, Merseyside, England
Univ Liverpool Liverpool Sch Trop Med, Dept Clin Sci, Liverpool L3 5QA, Merseyside, England
Green Published, gold, Green Accepted
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