Blood Rheological Characterization of beta-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry
Por:
Mendez-Mora, L, Cabello-Fusares, M, Ferre-Torres, J, Riera-Llobet, C, Krishnevskaya, E, Trejo-Soto, C, Payan-Pernia, S, Hernandez-Rodriguez, I, Morales-Indiano, C, Alarcon, T, Vives-Corrons, JL and Hernandez-Machado, A
Publicada:
21 oct 2021
Ahead of Print:
21 oct 2021
Resumen:
The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (beta-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of beta-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, beta-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA).
Filiaciones:
Mendez-Mora, L:
Univ Barcelona, Dept Condensed Matter Phys, Barcelona, Spain
Cabello-Fusares, M:
Ctr Recerca Matemat, Barcelona, Spain
Ferre-Torres, J:
Univ Barcelona, Dept Condensed Matter Phys, Barcelona, Spain
Riera-Llobet, C:
Univ Barcelona, Dept Condensed Matter Phys, Barcelona, Spain
Krishnevskaya, E:
Josep Carreras Leukaemia Res Inst, Red Cell Pathol & Hematopoiet Disorders Rare Anem, Badalona, Spain
Trejo-Soto, C:
Pontificia Univ Catolica Chile, Inst Fis, Valparaiso, Chile
Payan-Pernia, S:
Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBIS CSIC, Hematol Dept, Red Blood Cell Disorders Unit, Seville, Spain
:
Germans Trias & Pujol Univ Hosp, Inst Catala Oncol, Hematol Serv, Badalona, Spain
:
Hosp Badalona Germans Trias & Pujol, Lab Clin Metropolitana Nord, Lab Med Dept, Badalona, Spain
Alarcon, T:
Ctr Recerca Matemat, Barcelona, Spain
Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain
Univ Autonoma Barcelona, Dept Matemat, Bellaterra, Spain
:
Josep Carreras Leukaemia Res Inst, Red Cell Pathol & Hematopoiet Disorders Rare Anem, Badalona, Spain
Hernandez-Machado, A:
Univ Barcelona, Dept Condensed Matter Phys, Barcelona, Spain
Ctr Recerca Matemat, Barcelona, Spain
Univ Barcelona, Inst Nanosci & Nanotechnol, Barcelona, Spain
Green Published, gold
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