Exploring the evolution and epidemiology of European CC1 MRSA-IV: tracking a multidrug- resistant community-associated meticillin-resistant Staphylococcus aureus clone
Por:
Earls, MR, Steinig, EJ, Monecke, S, Castruita, JAS, Simbeck, A, Schneider-Brachert, W, Vremera, T, Dorneanu, OS, Loncaric, I, Bes, M, Lacoma, A, Aymerich, CP, Wernery, U, Armengol-Porta, M, Blomfeldt, A, Duchene, S, Bartels, MD, Ehricht, R and Coleman, DC
Publicada:
1 jul 2021
Resumen:
This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCCmec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant S. aureus (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic similar to 5350 nt insertion in SCCmec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe.
Filiaciones:
Earls, MR:
Univ Dublin, Dublin Dent Univ Hosp, Div Oral Biosci, Microbiol Res Unit,Trinity Coll, Dublin, Ireland
Steinig, EJ:
Australian Inst Trop Hlth & Med, Townsville, Qld, Australia
Monecke, S:
Leibniz Inst Photon Technol IPHT, Jena, Germany
Tech Univ Dresden, Inst Med Microbiol & Hyg, Med Fac Carl Gustav Carus, Dresden, Germany
InfectoGnost Res Campus Jena, Jena, Germany
Castruita, JAS:
Copenhagen Univ Hosp, Dept Clin Microbiol, Hvidovre, Denmark
Simbeck, A:
Univ Hosp Regensburg, Inst Clin Microbiol & Hyg, Regensburg, Germany
Schneider-Brachert, W:
Univ Hosp Regensburg, Inst Clin Microbiol & Hyg, Regensburg, Germany
Vremera, T:
Univ Med & Pharm Grigore T Popa, Dept Prevent & Interdisciplinary Med, Microbiol Unit, Iasi, Romania
Dorneanu, OS:
Univ Med & Pharm Grigore T Popa, Dept Prevent & Interdisciplinary Med, Microbiol Unit, Iasi, Romania
Loncaric, I:
Univ Vet Med Vienna, Inst Microbiol, Dept Pathobiol, Vienna, Austria
Bes, M:
Hosp Civils Lyon, Inst Agents Infectieux, Ctr Natl Reference Staphylocoques, Lyon, France
:
Univ Autonoma Barcelona, Inst Invest Germans Trias & Pujol, Hosp Univ Germans Trias & Pujol, CIBER Enfermedades Resp,Microbiol Dept, Badalona, Spain
:
Univ Autonoma Barcelona, Inst Invest Germans Trias & Pujol, Hosp Univ Germans Trias & Pujol, CIBER Enfermedades Resp,Microbiol Dept, Badalona, Spain
Univ Utrecht, Univ Med Ctr Utrecht, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Wernery, U:
Cent Vet Res Lab, Dubai, U Arab Emirates
Armengol-Porta, M:
Dresden Municipal Hosp, Microbiol Dept, Dresden, Germany
Blomfeldt, A:
Akershus Univ Hosp, Dept Microbiol & Infect Control, Lorenskog, Norway
Duchene, S:
Univ Melbourne, Peter Doherty Inst Infect & Immun, Melbourne, Vic, Australia
Bartels, MD:
Copenhagen Univ Hosp, Dept Clin Microbiol, Hvidovre, Denmark
Ehricht, R:
Leibniz Inst Photon Technol IPHT, Jena, Germany
InfectoGnost Res Campus Jena, Jena, Germany
Friedrich Schiller Univ, Inst Phys Chem, Jena, Germany
Coleman, DC:
Univ Dublin, Dublin Dent Univ Hosp, Div Oral Biosci, Microbiol Res Unit,Trinity Coll, Dublin, Ireland
Green Accepted, Green Published, gold
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