Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona
Por:
Fernandez-Naval, C, Arando, M, Espasa, M, Anton, A, Fernandez-Huerta, M, Silgado, A, Pinatar, C, Zarzuela, F, Gonzalez-Lopez, JJ, Serra-Pladevall, J, Sulleiro, E, Pumarola, T, Vall-Mayans, M and Esperalba, J
Publicada:
1 sep 2021
Ahead of Print:
1 ago 2021
Resumen:
Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.
Tweetable abstract We successfully identified 11 allelic profiles of T. pallidum from 63 fully typed syphilis samples using the MLST methodology. Profile 1.3.1 was found in 56% of samples, all of them being macrolide resistant.
Filiaciones:
Fernandez-Naval, C:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Arando, M:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Sexually Transmitted Infect Unit Vall dHebron Dra, Carrer St Oleguer 17, Barcelona 08001, Spain
Espasa, M:
Corp Sanitaria Parc Tauli, Dept Microbiol, Parc Tauli 1, Barcelona 08208, Spain
Anton, A:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Fernandez-Huerta, M:
Hosp Univ Bellvitge, Dept Microbiol, Barcelona 08907, Spain
Silgado, A:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Pinatar, C:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Zarzuela, F:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Sexually Transmitted Infect Unit Vall dHebron Dra, Carrer St Oleguer 17, Barcelona 08001, Spain
Gonzalez-Lopez, JJ:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Serra-Pladevall, J:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Sulleiro, E:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Pumarola, T:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
:
Hosp Germans Trias i Pujol, Dept Microbiol, Carretera Canyet, Barcelona 08916, Spain
Esperalba, J:
Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
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