Evaluation of VIASURE Sexually Transmitted Diseases Real Time PCR Detection kit (CerTest Biotec) for the diagnostic of sexually transmitted infections
Por:
Lopez, CC, Sanchez, BR, Rivas, GF, Rodriguez, AH, Torres, AA, Llamas, GL and Andreu, LM
Publicada:
1 may 2021
Ahead of Print:
1 may 2021
Resumen:
Introduction: Sexually transmitted infections (STIs) are common in our environment, and trends have been increasing in the last few years. Different methods for STIs diagnosis have been applied by microbiology laboratories over years, but real-time PCR has improved this process. Our objective was to evaluate VIASURE Sexually Transmitted Diseases Real-Time PCR Detection kit (CerTestBiotec, S.L.) comparing with the real-time PCR technique used in our laboratory (Allplex (TM) STI7 Essential Assay, Seegene) which was considered as reference assay.
Methods: A total of 948 samples from different sites (vaginal, endocervical, urethral, rectal, pharyngeal swabs and urine samples) were analyzed from July to September 2018.
Results: A discordant result was obtained in 4.5% (43 samples). These discrepancies were mainly observed in threshold cycle (Ct) value next to the limit of detection. The k coefficient obtained shows a very high agreement between both methods with k values from 0.92 to 0.99.
Conclusions: VIASURE Sexually Transmitted Diseases Real-Time PCR Detection kit provides a very good correlation with Allplex STI7 and therefore, it's a good tool for the diagnostic of STIs. Positive results with Ct value obtained from 35 and low amplification signal should be applied with caution and should be interpreted based on the patient's clinical data. (C) 2020 Elsevier Espana, S.L.U. and Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica. All rights reserved.
Filiaciones:
Lopez, CC:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
Sanchez, BR:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Badalona, Spain
:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Badalona, Spain
:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
Llamas, GL:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
:
Hosp Badalona Germans Trias & Pujol, Serv Microbiol, Lab Clin Metropolitana Norte, Badalona, Spain
Univ Autonoma Barcelona, Dept Genet & Microbiol, Badalona, Spain
CIBER Epidemiol & Salud Publ CIBERESP, Madrid, Spain
|