Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Por:
Royo, F, Azkargorta, M, Lavin, JL, Clos-Garcia, M, Cortazar, AR, Gonzalez-Lopez, M, Barcena, L, del Portillo, HA, Yanez-Mo, M, Marcilla, A, Borras, FE, Peinado, H, Guerrero, I, Vales-Gomez, M, Cereijo, U, Sardon, T, Aransay, AM, Elortza, F and Falcon-Perez, JM
Publicada:
12 ene 2021
Ahead of Print:
12 ene 2021
Resumen:
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.
Filiaciones:
Royo, F:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain
Azkargorta, M:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Lavin, JL:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Clos-Garcia, M:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Cortazar, AR:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Canc, Madrid, Spain
Gonzalez-Lopez, M:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Barcena, L:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
:
Univ Barcelona, ISGlobal, Hosp Clin, Barcelona, Spain
Hlth Sci Res Inst Germans Trias & Pujol, Badalona, Spain
Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain
Yanez-Mo, M:
Univ Autonoma Madrid, Inst Invest Sanit Princesa, Ctr Biol Mol Severo Ochoa, Dept Biol Mol, Madrid, Spain
Marcilla, A:
Univ Valencia, Area Parasitol, Dept Farm & Tecnol Farmaceut & Parasitol, Valencia, Spain
Univ Valencia, Hlth Res Inst Le Fe, Joint Res Unit Endocrinol Nutr & Clin Dietet, Valencia, Spain
:
Autonomous Univ Barcelona, Dept Cell Biol Physiol & Immunol, Barcelona, Spain
Germans Trias & Pujol Hlth Sci Res Inst IGTP, REMAR IVECAT Grp, Badalona, Spain
Germans Trias & Pujol Univ Hosp, Nephrol Dept, Can Ruti Campus, Badalona, Spain
Peinado, H:
Spanish Natl Canc Res Ctr, Mol Oncol Program, Microenvironm & Metastasis Lab, Madrid, Spain
Guerrero, I:
Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, Tissue & Organ Homeostasis, Madrid, Spain
Vales-Gomez, M:
Spanish Natl Res Council, Spanish Natl Ctr Biotechnol, Madrid, Spain
Cereijo, U:
Anax Biotech, Barcelona, Spain
Sardon, T:
Anax Biotech, Barcelona, Spain
Aransay, AM:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain
Elortza, F:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain
Falcon-Perez, JM:
Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain
Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain
Basque Fdn Sci, IKERBASQUE, Bilbao, Spain
gold, Green Published
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