Evaluation of Fourier Transform Infrared Spectroscopy as a First-Line Typing Tool for the Identification of Extended-Spectrum beta-Lactamase-Producing Klebsiella pneumoniae Outbreaks in the Hospital Setting
Por:
Wang-Wang, JH, Bordoy, AE, Martro, E, Quesada, MD, Perez-Vazquez, M, Guerrero-Murillo, M, Tiburcio, A, Navarro, M, Castella, L, Sopena, N, Casas, I, Saludes, V, Gimenez, M and Cardona, PJ
Publicada:
9 jun 2022
Resumen:
Early detection of pathogen cross-transmission events and environmental reservoirs is needed to control derived nosocomial outbreaks. Whole-genome sequencing (WGS) is considered the gold standard for outbreak confirmation, but, in most cases, it is time-consuming and has elevated costs. Consequently, the timely incorporation of WGS results to conventional epidemiology (CE) investigations for rapid outbreak detection is scarce. Fourier transform infrared spectroscopy (FTIR) is a rapid technique that establishes similarity among bacteria based on the comparison of infrared light absorption patterns of bacterial polysaccharides and has been used as a typing tool in recent studies. The aim of the present study was to evaluate the performance of the FTIR as a first-line typing tool for the identification of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-Kp) outbreaks in the hospital setting in comparison with CE investigations using WGS as the gold standard method. Sixty-three isolates of ESBL-Kp collected from 2018 to 2021 and classified according to CE were typed by both FTIR and WGS. Concordance was measured using the Adjusted Rand index (AR) and the Adjusted Wallace coefficient (AW) for both CE and FTIR clustering considering WGS as the reference method. Both AR and AW were significantly higher for FTIR clustering than CE clustering (0.475 vs. 0.134, p = 0.01, and 0.521 vs. 0.134, p = 0.009, respectively). Accordingly, FTIR inferred more true clustering relationships than CE (38/42 vs. 24/42, p = 0.001). However, a similar proportion of genomic singletons was detected by both FTIR and CE (13/21 vs. 12/21, p = 1). This study demonstrates the utility of the FTIR method as a quick, low-cost, first-line tool for the detection of ESBL-Kp outbreaks, while WGS analyses are being performed for outbreak confirmation and isolate characterization. Thus, clinical microbiology laboratories would benefit from integrating the FTIR method into CE investigations for infection control measures in the hospital setting.
Filiaciones:
Wang-Wang, JH:
Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Univ Autonoma Barcelona, Genet & Microbiol Dept, Bellaterra, Spain
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Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Germans Trias & Pujol Res Inst IGTP, Badalona, Spain
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Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Univ Autonoma Barcelona, Genet & Microbiol Dept, Bellaterra, Spain
Germans Trias & Pujol Res Inst IGTP, Badalona, Spain
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Epidemiol & Salud Publ CIBER, Madrid, Spain
:
Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Univ Autonoma Barcelona, Genet & Microbiol Dept, Bellaterra, Spain
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Epidemiol & Salud Publ CIBER, Madrid, Spain
Perez-Vazquez, M:
Inst Salud Carlos III ISCIII, Reference & Res Lab Antibiot Resistance & Hlth Car, Natl Ctr Microbiol, Madrid, Spain
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Enfermedades Infecciosas CIB, Madrid, Spain
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Germans Trias & Pujol Res Inst IGTP, Clin Genom Res Unit, Can Ruti Campus, Badalona, Spain
Germans Trias & Pujol Univ, Hosp, Lab Clin Metropolitana Nord,Clin Genet Serv, Clin Genom Unit, Can Ruti Campus, Badalona, Spain
Tiburcio, A:
Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Navarro, M:
Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
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Germans Trias & Pujol Univ, Hosp, Enfermeria Control Infecc, Direcc Enfermeria, Badalona, Spain
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Germans Trias & Pujol Univ, Hosp, Infect Dis Dept, Badalona, Spain
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Germans Trias & Pujol Univ, Hosp, Prevent Med Dept, Badalona, Spain
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Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Univ Autonoma Barcelona, Genet & Microbiol Dept, Bellaterra, Spain
Germans Trias & Pujol Res Inst IGTP, Badalona, Spain
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Epidemiol & Salud Publ CIBER, Madrid, Spain
:
Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Epidemiol & Salud Publ CIBER, Madrid, Spain
:
Germans Trias & Pujol Univ, Hosp, Microbiol Dept, Lab Clin Metropolitana Nord, Badalona, Spain
Univ Autonoma Barcelona, Genet & Microbiol Dept, Bellaterra, Spain
Germans Trias & Pujol Res Inst IGTP, Badalona, Spain
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Enfermedades Respiratorias C, Madrid, Spain
gold, Green Published
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