Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model
Por:
Baez-Diaz, C, Blanco-Blazquez, V, Sanchez-Margallo, FM, Bayes-Genis, A, Gonzalez, I, Abad, A, Steendam, R, Franssen, O, Palacios, I, Sanchez, B, Galvez-Monton, C and Crisostomo, V
Publicada:
28 abr 2020
Ahead of Print:
28 abr 2020
Resumen:
Insulin-like growth factor-1 (IGF-1) has demonstrated beneficial effects after myocardial infarction (MI). Microencapsulation of IGF-1 could potentially improve results. We aimed to test the effect of an intracoronary (IC) infusion of microencapsulated IGF-1 in a swine acute MI model. For that purpose IC injection of a 10ml solution of 5 x 10(6) IGF-1 loaded microspheres (MSPs) (n=8, IGF-1 MSPs), 5 x 10(6) unloaded MSPs (n=9; MSPs) or saline (n=7; CON) was performed 48hours post-MI. Left ventricular ejection fraction (LVEF), indexed ventricular volumes and infarct size (IS) were determined by cardiac magnetic resonance at pre-injection and 10 weeks. Animals were euthanized at 10 weeks, and myocardial fibrosis and vascular density were analysed. End-study LVEF was significantly greater in IGF-1 MSPs compared to MSPs and CON, while ventricular volumes exhibited no significant differences between groups. IS decreased over time in all groups. Collagen volume fraction on the infarct area was significantly reduced in IGF-1 MSPs compared to CON and MSPs. Vascular density analysis of infarct and border zones showed no significant differences between groups. In conclusion, the IC injection of 5 x 10(6) IGF-1 loaded MSPs in a porcine acute MI model successfully improves cardiac function and limits myocardial fibrosis, which could be clinically relevant.
Filiaciones:
Baez-Diaz, C:
Jesus Uson Minimally Invas Surg Ctr, Caceres, Spain
CIBERCV, Madrid, Spain
Blanco-Blazquez, V:
Jesus Uson Minimally Invas Surg Ctr, Caceres, Spain
CIBERCV, Madrid, Spain
Sanchez-Margallo, FM:
Jesus Uson Minimally Invas Surg Ctr, Caceres, Spain
CIBERCV, Madrid, Spain
:
CIBERCV, Madrid, Spain
Hlth Sci Res Inst Germans Trias & Pujol IGTP, ICREC Heart Failure & Cardiac Regenerat Res Progr, Barcelona, Spain
Gonzalez, I:
Jesus Uson Minimally Invas Surg Ctr, Caceres, Spain
Abad, A:
Jesus Uson Minimally Invas Surg Ctr, Caceres, Spain
Steendam, R:
Innocore Pharmaceut, Groningen, Netherlands
Franssen, O:
Nanomi BV, Oldenzaal, Netherlands
Palacios, I:
Tigenix, Madrid, Spain
Sanchez, B:
Tigenix, Madrid, Spain
:
CIBERCV, Madrid, Spain
Hlth Sci Res Inst Germans Trias & Pujol IGTP, ICREC Heart Failure & Cardiac Regenerat Res Progr, Barcelona, Spain
Crisostomo, V:
Jesus Uson Minimally Invas Surg Ctr, Caceres, Spain
CIBERCV, Madrid, Spain
gold, Green Published
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