UHPLC-HRMS and GC-MS Screening of a Selection of Synthetic Cannabinoids and Metabolites in Urine of Consumers


Por: Pellegrini, M, Marchei, E, Papaseit, E, Farre, M and Zaami, S

Publicada: 1 ago 2020 Ahead of Print: 13 ago 2020
Resumen:
Background and Objectives: The use of synthetic cannabinoids has increased around the world. As a result, the implementation of accurate analysis in human biological matrices is relevant and fundamental. Two different analytical technologies, ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and high-sensitivity gas chromatography-mass spectrometry (GC-MS) were used for the determination of three synthetic cannabinoids JWH-122, JWH 210, UR-144 and their metabolites in urine of consumers.Materials and Methods: Sample preparation included an initial hydrolysis with beta-glucuronidase and liquid-liquid extraction. The UHPLC-HRMS method included a Kinetex 2.6 u Biphenyl 100A (100 x 2.1 mm, 2.6 mu m) (Phenomenex, Italy) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode was used (mass range 100-1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m x 250 mu m i.d, film thickness 0.25 mu m; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40-550 m/z).Results:Both methods have been successfully used for screening of parent synthetic cannabinoids and their metabolites in urine samples of consumers.Conclusions:The screening method applied JWH-122, JWH-210, UR-144 and their metabolites in urine of consumers can be applied to other compounds of the JWH family.

Direcciones
Pellegrini, M: Ist Super Sanita, Natl Ctr Addict & Doping, VLe Regina Elena 299, I-00161 Rome, Italy
Marchei, E: Ist Super Sanita, Natl Ctr Addict & Doping, VLe Regina Elena 299, I-00161 Rome, Italy
Papaseit, E: Hosp Badalona Germans Trias & Pujol, Clin Pharmacol Unit, Badalona 08916, Spain; Inst Recerca Germans Trias & Pujol HUGTiP IGTP, Badalona 08916, Spain; Univ Autonoma Barcelona, Dept Pharmacol Therapeut & Toxicol, Cerdanyola Del Valles 08193, Spain
Farre, M: Hosp Badalona Germans Trias & Pujol, Clin Pharmacol Unit, Badalona 08916, Spain; Inst Recerca Germans Trias & Pujol HUGTiP IGTP, Badalona 08916, Spain; Univ Autonoma Barcelona, Dept Pharmacol Therapeut & Toxicol, Cerdanyola Del Valles 08193, Spain
Zaami, S: Sapienza Univ, Dept Anat Histol Forens & Orthoped Sci, I-00161 Rome, Italy
ISSN: 16489144





MEDICINA-LITHUANIA
Editorial
Kauno Medicinos Universitetas, ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND, Lituania
Tipo de documento: Article
Volumen: 56 Número: 8
Páginas:
WOS Id: 000564207800001
ID de PubMed: 32823724
imagen Green Published, gold

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