Extracellular vesicles derived from Plasmodium-infected and non-infected red blood cells as targeted drug delivery vehicles
Por:
Borgheti-Cardoso, LN, Kooijmans, SAA, Chamorro, LG, Biosca, A, Lantero, E, Ramirez, M, Avalos-Padilla, Y, Crespo, I, Fernandez, I, Fernandez-Becerra, C, del Portillo, HA and Fernandez-Busquets, X
Publicada:
25 sep 2020
Ahead of Print:
9 jul 2020
Resumen:
Among several factors behind drug resistance evolution in malaria is the challenge of administering overall doses that are not toxic for the patient but that, locally, are sufficiently high to rapidly kill the parasites. Thus, a crucial antimalarial strategy is the development of drug delivery systems capable of targeting antimalarial compounds to Plasmodium with high specificity. In the present study, extracellular vesicles (EVs) have been evaluated as a drug delivery system for the treatment of malaria. EVs derived from naive red blood cells (RBCs) and from Plasmodium falciparum-infected RBCs (pRBCs) were isolated by ultrafiltration followed by size exclusion chromatography. Lipidomic characterization showed that there were no significant qualitative differences between the lipidomic profiles of pRBC-derived EVs (pRBC-EVs) and RBC-derived EVs (RBC-EVs). Both EVs were taken up by RBCs and pRBCs, although pRBC-EVs were more efficiently internalized than RBC-EVs, which suggested their potential use as drug delivery vehicles for these cells. When loaded into pRBC-EVs, the antimalarial drugs atovaquone and tafenoquine inhibited in vitro P. falciparum growth more efficiently than their free drug counterparts, indicating that pRBC-EVs can potentially increase the efficacy of several small hydrophobic drugs used for the treatment of malaria.
Filiaciones:
Borgheti-Cardoso, LN:
Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Barcelona, Spain
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Kooijmans, SAA:
Univ Med Ctr Utrecht, CDL Res, Utrecht, Netherlands
Chamorro, LG:
Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Barcelona, Spain
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Biosca, A:
Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Barcelona, Spain
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Lantero, E:
Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Barcelona, Spain
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Ramirez, M:
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Avalos-Padilla, Y:
Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Barcelona, Spain
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Crespo, I:
Inst Invest Biomed August i Pi Sunyer IDIBAPS, Plataforma Citometria, Barcelona, Spain
Fernandez, I:
Univ Barcelona UB, Unitat Espectrometria Masses Caracteritzacio Mol, CCiTUB, Barcelona, Spain
Fernandez-Becerra, C:
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Inst Invest Ciencies Salut Germans Trias & Pujol, Badalona, Spain
:
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
Inst Invest Ciencies Salut Germans Trias & Pujol, Badalona, Spain
Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain
Fernandez-Busquets, X:
Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Barcelona, Spain
Hosp Clin Univ Barcelona, Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain
UB, Nanosci & Nanotechnol Inst IN2UB, Barcelona, Spain
Green Accepted
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