Laboratory sample stability. Is it possible to define a consensus stability function? An example of five blood magnitudes
Por:
Rioja, RG, Espartosa, DM, Segovia, M, Ibarz, M, Llopis, MA, Bauca, JM, Marzana, I, Barba, N, Ventura, M, del Pino, IG, Puente, JJ, Caballero, A, Gomez, C, Alvarez, AG, Alsina, MJ and Alvarez, V
Publicada:
1 nov 2018
Resumen:
Background: The stability limit of an analyte in a biological sample can be defined as the time required until a measured property acquires a bias higher than a defined specification. Many studies assessing stability and presenting recommendations of stability limits are available, but differences among them are frequent. The aim of this study was to classify and to grade a set of bibliographic studies on the stability of five common blood measurands and subsequently generate a consensus stability function.
Methods: First, a bibliographic search was made for stability studies for five analytes in blood: alanine aminotransferase (ALT), glucose, phosphorus, potassium and prostate specific antigen (PSA). The quality of every study was evaluated using an in-house grading tool. Second, the different conditions of stability were uniformly defined and the percent deviation (PD%) over time for each analyte and condition were scattered while unifying studies with similar conditions.
Results: From the 37 articles considered as valid, up to 130 experiments were evaluated and 629 PD% data were included (106 for ALT, 180 for glucose, 113 for phosphorus, 145 for potassium and 85 for PSA). Consensus stability equations were established for glucose, potassium, phosphorus and PSA, but not for ALT.
Conclusions: Time is the main variable affecting stability in medical laboratory samples. Bibliographic studies differ in recommedations of stability limits mainly because of different specifications for maximum allowable error. Definition of a consensus stability function in specific conditions can help laboratories define stability limits using their own quality specifications.
Filiaciones:
Rioja, RG:
La Paz Cantoblanco Carlos III Univ Hosp, Lab Med, Madrid, Spain
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Espartosa, DM:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Clin Univ Navarra, Lab Med, Madrid, Spain
Segovia, M:
La Paz Cantoblanco Carlos III Univ Hosp, Lab Med, Madrid, Spain
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Ibarz, M:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Arnau Vilanova, Lab Clin Terr ICS, IRB Lleida, Lleida, Catalunya, Spain
:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Badalona Germans Trias & Pujol, Lab Med, Badalona, Spain
Bauca, JM:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Univ Son Espases, Dept Lab Med, Palma De Mallorca, Illes Balears, Spain
Marzana, I:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Univ Cruces Lab, Baracaldo, Pais Vasco, Spain
Barba, N:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
CatLab, Barcelona, Spain
Ventura, M:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
del Pino, IG:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Complexo Hosp Univ A Coruna, Area Lab, La Coruna, Spain
Puente, JJ:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Clin Univ Lozano Blesa, Serv Bioquim, Zaragoza, Spain
Caballero, A:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Valle De Hebron, Serv Bioquim, Barcelona, Catalunya, Spain
:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Badalona Germans Trias & Pujol, Lab Med, Badalona, Spain
Alvarez, AG:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Hosp Clin Univ San Carlos, Madrid, Spain
Alsina, MJ:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Alvarez, V:
Comis Calidad Extra Analit, Soc Espanola, Med Lab, Barcelona, Spain
Green Published
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