Epigenome-wide analysis reveals specific DNA hypermethylation of T cells during human hematopoietic differentiation


Por: Tejedor, JR, Bueno, C, Cobo, I, Bayon, GF, Prieto, C, Mangas, C, Perez, RF, Santamarina, P, Urdinguio, RG, Menendez, P, Fraga, MF and Fernandez, AF

Publicada: 1 jul 2018
Resumen:
Aim: Epigenetic regulation plays an important role in cellular development and differentiation. A detailed map of the DNA methylation dynamics that occur during cell differentiation would contribute to decipher the molecular networks governing cell fate commitment. Methods: Illumina Methylation EPIC BeadChip platform was used to describe the genome-wide DNA methylation changes observed throughout hematopoietic maturation by analyzing multiple myeloid and lymphoid hematopoietic cell types. Results: We identified a plethora of DNA methylation changes that occur during human hematopoietic differentiation. We observed that T lymphocytes display substantial enhancement of de novo CpG hypermethylation as compared with other hematopoietic cell populations. T-cell-specific hypermethylated regions were strongly associated with open chromatin marks and enhancer elements, as well as binding sites of specific key transcription factors involved in hematopoietic differentiation, such as PU. 1 and TAL1. Conclusion: These results provide novel insights into the role of DNA methylation at enhancer elements in T-cell development.

Filiaciones:
Tejedor, JR:
 Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain

 Fdn Invest Biosanitaria Asturias FINBA, Inst Invest Sanitaria Principado de Asturias, Principado De Asturias, Spain

:
 Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain

Cobo, I:
 Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain

Bayon, GF:
 Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain

Prieto, C:
 Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain

Mangas, C:
 Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain

Perez, RF:
 Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain

 Univ Oviedo, Nanomat & Nanotechnol Res Ctr CINN CSIC, Principado De Asturias, Spain

Santamarina, P:
 Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain

 Fdn Invest Biosanitaria Asturias FINBA, Inst Invest Sanitaria Principado de Asturias, Principado De Asturias, Spain

Urdinguio, RG:
 Univ Oviedo, Nanomat & Nanotechnol Res Ctr CINN CSIC, Principado De Asturias, Spain

:
 Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain

 ICREA, Barcelona, Spain

 Res Inst Germans Trias & Pujol IGTP, Josep Carreras Leukemia Res Inst Campus ICO, Badalona, Spain

 ISCIII, Ctr Invest Biomed Red Canc CIBERONC, Barcelona, Spain

Fraga, MF:
 Univ Oviedo, Nanomat & Nanotechnol Res Ctr CINN CSIC, Principado De Asturias, Spain

Fernandez, AF:
 Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain

 Fdn Invest Biosanitaria Asturias FINBA, Inst Invest Sanitaria Principado de Asturias, Principado De Asturias, Spain
ISSN: 17501911





Epigenomics
Editorial
Future Medicine Ltd., UNITEC HOUSE, 3RD FLOOR, 2 ALBERT PLACE, FINCHLEY CENTRAL, LONDON, N3 1QB, ENGLAND, Reino Unido
Tipo de documento: Article
Volumen: 10 Número: 7
Páginas: 903-923
WOS Id: 000438759200005
ID de PubMed: 29620943
imagen Green Submitted

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