Epigenome-wide analysis reveals specific DNA hypermethylation of T cells during human hematopoietic differentiation
Por:
Tejedor, JR, Bueno, C, Cobo, I, Bayon, GF, Prieto, C, Mangas, C, Perez, RF, Santamarina, P, Urdinguio, RG, Menendez, P, Fraga, MF and Fernandez, AF
Publicada:
1 jul 2018
Resumen:
Aim: Epigenetic regulation plays an important role in cellular development and differentiation. A detailed map of the DNA methylation dynamics that occur during cell differentiation would contribute to decipher the molecular networks governing cell fate commitment. Methods: Illumina Methylation EPIC BeadChip platform was used to describe the genome-wide DNA methylation changes observed throughout hematopoietic maturation by analyzing multiple myeloid and lymphoid hematopoietic cell types. Results: We identified a plethora of DNA methylation changes that occur during human hematopoietic differentiation. We observed that T lymphocytes display substantial enhancement of de novo CpG hypermethylation as compared with other hematopoietic cell populations. T-cell-specific hypermethylated regions were strongly associated with open chromatin marks and enhancer elements, as well as binding sites of specific key transcription factors involved in hematopoietic differentiation, such as PU. 1 and TAL1. Conclusion: These results provide novel insights into the role of DNA methylation at enhancer elements in T-cell development.
Filiaciones:
Tejedor, JR:
Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain
Fdn Invest Biosanitaria Asturias FINBA, Inst Invest Sanitaria Principado de Asturias, Principado De Asturias, Spain
:
Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain
Cobo, I:
Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain
Bayon, GF:
Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain
Prieto, C:
Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain
Mangas, C:
Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain
Perez, RF:
Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain
Univ Oviedo, Nanomat & Nanotechnol Res Ctr CINN CSIC, Principado De Asturias, Spain
Santamarina, P:
Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain
Fdn Invest Biosanitaria Asturias FINBA, Inst Invest Sanitaria Principado de Asturias, Principado De Asturias, Spain
Urdinguio, RG:
Univ Oviedo, Nanomat & Nanotechnol Res Ctr CINN CSIC, Principado De Asturias, Spain
:
Univ Barcelona, Dept Biomed, Sch Med, Josep Carreras Leukemia Res Inst, Barcelona, Spain
ICREA, Barcelona, Spain
Res Inst Germans Trias & Pujol IGTP, Josep Carreras Leukemia Res Inst Campus ICO, Badalona, Spain
ISCIII, Ctr Invest Biomed Red Canc CIBERONC, Barcelona, Spain
Fraga, MF:
Univ Oviedo, Nanomat & Nanotechnol Res Ctr CINN CSIC, Principado De Asturias, Spain
Fernandez, AF:
Univ Oviedo, Canc Epigenet Lab, Inst Oncol Asturias IUOPA, HUCA, Principado De Asturias, Spain
Fdn Invest Biosanitaria Asturias FINBA, Inst Invest Sanitaria Principado de Asturias, Principado De Asturias, Spain
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