Morphofunctional Changes After Sleeve Gastrectomy and Very Low Calorie Diet in an Animal Model of Non-Alcoholic Fatty Liver Disease
Por:
Talavera-Urquijo, E, Rodriguez-Navarro, S, Beisani, M, Salcedo-Allende, MT, Chakkur, A, Arus-Aviles, M, Cremades, M, Augustin, S, Martell, M and Balibrea, JM
Publicada:
1 ene 2018
Resumen:
Background Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease and is found in 70% of obese people. The evidence available to date suggests that bariatric surgery could be an effective treatment by reducing weight and also by improving metabolic complications in the long term. This work aimed to compare, in a diet-induced NAFLD animal model, the effect of both sleeve gastrectomy (SG) and very-low calorie diet (VLCD).
Methods Thirty-five Wistar rats were divided into control rats (n = 7) and obese rats fed a high-fat diet (HFD). After 10 weeks, the obese rats were subdivided into four groups: HFD (n = 7), VLCD (n = 7), and rats submitted to either a sham operation (n = 7) or SG (n = 7). Both liver tissue and blood samples were processed to evaluate steatosis and NASH changes in histology (Oil Red, Sirius Red and H & presence of endothelial damage (CD31, Moesin/p-Moesin, Akt/p-Akt, eNOS/p-eNOS), oxidative stress (iNOS) and fibrosis (alpha SMA, Col1, PDGF, VEGF) proteins in liver tissue; and inflammatory (IL6, IL10, MCP-1, IL17 alpha, TNF alpha), liver biochemical function, and hormonal (leptin, ghrelin, visfatin and insulin) alterations in plasma.
Results Both VLCD and SG improved histology, but only SG induced a significant weight loss, improved endothelial damage, and a decreased cardiovascular risk by reducing insulin resistance (IR), leptin, total cholesterol, and triglyceride levels. There were no relevant variations in the inflammatory and fibrosis markers.
Conclusion Our study suggests a slight superiority of SG over VLCD by improving not only the histology but also the IR and cardiovascular risk markers related to NAFLD.
Filiaciones:
Talavera-Urquijo, E:
Vall dHebron Univ Hosp, Dept Gen & Digest Surg, Barcelona, Spain
Rodriguez-Navarro, S:
Univ Autonoma Barcelona, Inst Recerca Vall dHebron VHIR, Hosp Univ Vall dHebron, Dept Internal Med,Liver Unit, Barcelona, Spain
Beisani, M:
Vall dHebron Univ Hosp, Dept Gen & Digest Surg, Barcelona, Spain
Salcedo-Allende, MT:
Vall dHebron Univ Hosp, Human Pathol Dept, Barcelona, Spain
Chakkur, A:
Univ Autonoma Barcelona, Inst Recerca Vall dHebron VHIR, Hosp Univ Vall dHebron, Dept Internal Med,Liver Unit, Barcelona, Spain
Arus-Aviles, M:
Univ Autonoma Barcelona, Inst Recerca Vall dHebron VHIR, Hosp Univ Vall dHebron, Dept Internal Med,Liver Unit, Barcelona, Spain
:
Germans Trias & Pujol Univ Hosp, Dept Gen & Digest Surg, Barcelona, Spain
Augustin, S:
Univ Autonoma Barcelona, Inst Recerca Vall dHebron VHIR, Hosp Univ Vall dHebron, Dept Internal Med,Liver Unit, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain
Martell, M:
Univ Autonoma Barcelona, Inst Recerca Vall dHebron VHIR, Hosp Univ Vall dHebron, Dept Internal Med,Liver Unit, Barcelona, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain
Balibrea, JM:
Vall dHebron Univ Hosp, Dept Gen & Digest Surg, Barcelona, Spain
Vall dHebron Univ Hosp, Metab & Bariatr Surg Unit, EAC BS Ctr Excellence, Passeig Vall dHebron 119,Edificio Gen, Barcelona 08035, Spain
Univ Autonoma Barcelona, Dept Surg, Barcelona, Spain
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