Immunophenotypic, Cytogenetic, and Mutational Characterization of Cell Lines Derived from Myelodysplastic Syndrome Patients After Progression to Acute Myeloid Leukemia
Por:
Palau, A, Mallo, M, Palomo, L, Rodriguez-Hernandez, I, Diesch, J, Campos, D, Granada, I, Junca, J, Drexler, HG, Francesc Solé and Buschbeck, M
Publicada:
1 mar 2017
Resumen:
Leukemia cell lines have been widely used in the hematology field to unravel mechanistic insights and to test new therapeutic strategies. Myelodysplastic syndromes (MDS) comprise a heterogeneous group of diseases that are characterized by ineffective hematopoiesis and frequent progress to acute myeloid leukemia (AML). A few cell lines have been established from MDS patients after progression to AML but their characterization is incomplete. Here we provide a detailed description of the immunophenotypic profile of the MDS-derived cell lines SKK-1, SKM-1, F-36P; and MOLM-13. Specifically, we analyzed a comprehensive panel of markers that are currently applied in the diagnostic routine for myeloid disorders. To provide high-resolution genetic data comprising copy number alterations and losses of heterozygosity we performed whole genome single nucleotide polymorphism-based arrays and included the cell line OHN-GM that harbors the frequent chromosome arm 5q deletion. Furthermore, we assessed the mutational status of 83 disease-relevant genes. Our results provide a resource to the MDS and AML field that allows researchers to choose the best-matching cell line for their functional studies. (C) 2016 Wiley Periodicals, Inc.
Filiaciones:
Palau, A:
Josep Carreras Leukaemia Res Inst, Campus ICO Germans Trias & Pujol, Badalona, Spain
:
Josep Carreras Leukaemia Res Inst, Campus ICO Germans Trias & Pujol, Badalona, Spain
:
Josep Carreras Leukaemia Res Inst, Campus ICO Germans Trias & Pujol, Badalona, Spain
Rodriguez-Hernandez, I:
Univ Autonoma Barcelona, Josep Carreras Leukemia Res Inst, ICO Hosp Germans Trias & Pujol, Dept Clin Hematol, Badalona, Spain
:
Josep Carreras Leukaemia Res Inst, Campus ICO Germans Trias & Pujol, Badalona, Spain
Campos, D:
Univ Autonoma Barcelona, Josep Carreras Leukemia Res Inst, ICO Hosp Germans Trias & Pujol, Dept Clin Hematol, Badalona, Spain
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Univ Autonoma Barcelona, Josep Carreras Leukemia Res Inst, ICO Hosp Germans Trias & Pujol, Dept Clin Hematol, Badalona, Spain
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Univ Autonoma Barcelona, Josep Carreras Leukemia Res Inst, ICO Hosp Germans Trias & Pujol, Dept Clin Hematol, Badalona, Spain
Drexler, HG:
German Collect Microorganisms & Cell Cultures, Leibniz Inst DSMZ, Dept Human & Anim Cell Lines, Braunschweig, Germany
Francesc Solé:
Josep Carreras Leukaemia Res Inst, Campus ICO Germans Trias & Pujol, Badalona, Spain
:
Josep Carreras Leukaemia Res Inst, Campus ICO Germans Trias & Pujol, Badalona, Spain
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