Using human artificial chromosomes to study centromere assembly and function
Por:
Molina, O, Kouprina, N, Masumoto, H, Larionov, V and Earnshaw, WC
Publicada:
1 oct 2017
Resumen:
Centromeres are the site of assembly of the kinetochore, which directs chromosome segregation during cell division. Active centromeres are characterized by the presence of nucleosomes containing CENP-A and a specific chromatin environment that resembles that of active genes. Recent work using human artificial chromosomes (HAC) sheds light on the fine balance of different histone post-translational modifications and transcription that exists at centromeres for kinetochore assembly and maintenance. Here, we review the use of HAC technology to understand centromere assembly and function. We put particular emphasis on studies using the alphoidtetO HAC, whose centromere can be specifically modified for epigenetic engineering studies.
Filiaciones:
:
Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3QR, Midlothian, Scotland
Univ Barcelona, Sch Med, Josep Carreras Leukaemia Res Inst, Casanova 143, E-08036 Barcelona, Spain
Kouprina, N:
NCI, Genome Struct & Funct Grp, Dev Therapeut Branch, NIH, Bethesda, MD 20892 USA
Masumoto, H:
Kazusa DNA Res Inst, Dept Frontier Res, Lab Cell Engn, Kisarazu 2920818, Japan
Larionov, V:
NCI, Genome Struct & Funct Grp, Dev Therapeut Branch, NIH, Bethesda, MD 20892 USA
Earnshaw, WC:
Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3QR, Midlothian, Scotland
Green Accepted
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