Postinfarction Functional Recovery Driven by a Three-Dimensional Engineered Fibrin Patch Composed of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells
Por:
Roura, S, Soler-Botija, C, Bago, JR, Llucia-Valldeperas, A, Fernandez, MA, Galvez-Monton, C, Prat-Vidal, C, Perea-Gil, I, Blanco, J and Bayes-Genis, A
Publicada:
1 ago 2015
Resumen:
Considerable research has been dedicated to restoring myocardial cell slippage and limiting ventricular remodeling after myocardial infarction (MI). We examined the ability of a three-dimensional (3D) engineered fibrin patch filled with human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) to induce recovery of cardiac function after MI. The UCBMSCs were modified to coexpress luciferase and fluorescent protein reporters, mixed with fibrin, and applied as an adhesive, viable construct (fibrin-cell patch) over the infarcted myocardium in mice (MI-UCBMSC group). The patch adhered well to the heart. Noninvasive bioluminescence imaging demonstrated early proliferation and differentiation of UCBMSCs within the construct in the postinfarct mice in the MI-UCBMSC group. The implanted cells also participated in the formation of new, functional microvasculature that connected the fibrin-cell patch to both the subjacent myocardial tissue and the host circulatory system. As revealed by echocardiography, the left ventricular ejection fraction and fractional shortening at sacrifice were improved in MI-UCBMSC mice and were markedly reduced in mice treated with fibrin alone and untreated postinfarction controls. In conclusion, a 3D engineered fibrin patch composed of UCBMSCs attenuated infarct-derived cardiac dysfunction when transplanted locally over a myocardial wound.
Filiaciones:
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Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
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Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
Bago, JR:
Inst Adv Chem Catalonia, Cell Therapy Grp, Barcelona, Spain
Biomed Res Networking Ctr Bioengn Biomat & Nanome, Zaragoza, Spain
Llucia-Valldeperas, A:
Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
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Germans Trias & Pujol Hlth Sci Res Inst, Flow Cytometry Facil, Badalona, Spain
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Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
Prat-Vidal, C:
Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
Perea-Gil, I:
Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
Blanco, J:
Inst Adv Chem Catalonia, Cell Therapy Grp, Barcelona, Spain
Biomed Res Networking Ctr Bioengn Biomat & Nanome, Zaragoza, Spain
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Germans Trias & Pujol Hlth Sci Res Inst, Heart Failure & Cardiac Regenerat Res Program, Badalona, Spain
Univ Hosp, Serv Cardiol, Badalona, Spain
Univ Autonoma Barcelona, Dept Med, E-08193 Barcelona, Spain
Green Submitted, Green Published, gold
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