The tuberculin skin test increases the responses measured by T cell interferon-gamma release assays
Por:
Vilaplana, C, Ruiz-Manzano, J, Gil, O, Cuchillo, F, Montane, E, Singh, M, Spallek, R, Ausina, V and Cardona, PJ
Publicada:
1 jun 2008
Resumen:
RUTI is a vaccine consisting of Mycobacterium tuberculosis bacilli grown in stress conditions that is fragmented, detoxified and liposomed. RUTI was designed to shorten the treatment of latent tuberculosis infection (LTBI) with isoniazid from 9 months to just 1 month, by additional treatment with two inoculations of RUTI 4 weeks apart. During the validation process for monitoring the immunogenicity of administration of RUTI in a Phase I clinical trial, the question arose whether to introduce the tuberculin skin test (TST) in the screening of non-LTBI volunteers. This study was designed to evaluate the effect of TST on subsequent different T-cell interferon-gamma release assay (TIGRA) responses, using a spectrum of M. tuberculosis-related antigens (ESAT-6, CFP-10, 16 kDa, 19 kDa, MPT64, Ag 85B, 38 kDa, hsp65, PPD and BCG). The results showed an increase in post-TST response even in non-LTBI subjects for most antigens tested, as measured both by whole blood assay (WBA) and ELISPOT. Increased ELISPOT response decreased toward pre-TST levels within 1 month whereas the WBA response did not. Taking into account that there is no definitive correlation between TST and TIGRA tests to diagnose LTBI and the feasibility that TST might alter the immune monitoring included in clinical trials, these data suggest that TST determination should be carefully planned to avoid any interference with TIGRA.
Filiaciones:
:
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Unitat TB Expt, Badalona 08916, Catalonia, Spain
CIBERES, Barcelona, Spain
Gil, O:
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Unitat TB Expt, Badalona 08916, Catalonia, Spain
CIBERES, Barcelona, Spain
Cuchillo, F:
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Unitat TB Expt, Badalona 08916, Catalonia, Spain
CIBERES, Barcelona, Spain
:
Hosp Badalona Germans Trias & Pujol, Dept Pharmacol, Badalona 08916, Catalonia, Spain
Singh, M:
Lionex Diagnost & Therapeut GMBH, Braunschweig, Germany
Spallek, R:
Lionex Diagnost & Therapeut GMBH, Braunschweig, Germany
Ausina, V:
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Unitat TB Expt, Badalona 08916, Catalonia, Spain
CIBERES, Barcelona, Spain
:
Univ Autonoma Barcelona, Fundacio Inst Invest Ciencies Salut Germans Trias, Unitat TB Expt, Badalona 08916, Catalonia, Spain
CIBERES, Barcelona, Spain
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